The pneumatic tourniquet is widely used in orthopedic surgery; however, it may causea series of complications. Some investigators have used ultrasound-guided methods tofind lower tourniquet pressures that permit the operation to proceed while mitigatingcomplications. Studies have shown that a tourniquet can damage patients� pulmonaryventilation function. It remains unclear, however, whether setting the pressure of thetourniquet under the guidance of ultrasound can reduce injury to pulmonaryventilation function in patients undergoing lower limb surgery. A total of 100 patientswho required tourniquet for lower limb surgery were divided into two groups (n = 50each): control and ultrasound-guided. In the ultrasound-guidance group, the optimaltourniquet pressure was the pressure at which popliteal artery blood flow disappearedon imaging. Pneumatic tourniquet pressure was set using traditional methods in thecontrol group. At specified time points (immediately after tourniquet inflation [T0], 1h after tourniquet inflation [T1], and 0.5 h [T2], 2 h [T3], 6 h [T4] and 24 h [T5] aftertourniquet deflation), several parameters were measured, including mean arterialpressure (MAP), heart rate (HR), plasma malondialdehyde (MDA) and superoxidedismutase (SOD) concentration, partial pressure of arterial blood oxygen (PaO2),partial pressure of arterial CO2 (PaCO2), and calculated respiratory index (RI),alveolar artery blood oxygen partial pressure difference (PA�aDO2) and arterialblood/alveolar oxygen partial pressure ratio (A/a ratio). In both groups, MAP waslower, and HR was more rapid at T2 than at T0. Compared with T0, PaO2 and the a/Aratio decreased, and PA-aDO2 and RI ratio increased at T4 in both groups. Comparedwith T0, the concentration of MDA was higher and the concentration of SOD waslower at T3�T5 in the control group. In the ultrasound-guided group, theconcentration of MDA was higher and the concentration of SOD was lower at T3�T5than at T0. Compared with the control group, in the ultrasound-guided group MAPincreased and HR decreased at T2?the ratio of PaO2 and a/A increased, while the ratioof PA-aDO2 and RI decreased at T4 and the concentration of MDA decreasedsignificantly, the concentration of SOD increased significantly at T3�T5. Therefore,patients in whom tourniquet pressure was set using ultrasound exhibited more stableMAP and HR, and less dysfunction in pulmonary ventilation compared with thetraditional method of pressure setting. A possible mechanism is that this methodreduces the consumption of the oxygen free radical scavenging agent (SOD), thusreducing lipid peroxide reactions (MDA) and reduces lipid peroxide injury in theprocess of limb ischemia-reperfusion injury caused by the tourniquet.